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In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel

Paper ID Volume ID Publish Year Pages File Format Full-Text
12707 813 2004 8 PDF Available
Title
In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel
Abstract

There is a clinical need for synthetic scaffolds that promote bone regeneration. A common problem encountered when using scaffolds in tissue engineering is the rapid formation of tissue on the outer edge of the scaffold whilst the tissue in the centre becomes necrotic. To address this, the scaffold design should improve nutrient and cell transfer to the scaffold centre. In this study, hydroxyapatite scaffolds with random, open porosity (average pore size of 282±11 μm, average interconnecting window size of 72±4 μm) were manufactured using a modified slip-casting methodology with a single aligned channel inserted into the centre. By varying the aligned channel diameter, a series of scaffolds with channel diameters ranging from 170 to 421 μm were produced. These scaffolds were seeded with human osteosarcoma (HOS TE85) cells and cultured for 8 days. Analysis of cell penetration into the aligned channels revealed that cell coverage increased with increasing channel diameter; from 22±3% in the 170 μm diameter channel to 38±6% coverage in the 421 μm channel. Cell penetration into the middle section of the 421 μm diameter channel (average cell area coverage 121×103±32×103 μm2) was significantly greater than that observed within the 170 μm channel (average cell area coverage 26×103±6×103 μm2). In addition, the data presented demonstrates that the minimum channel (or pore) diameter required for cell penetration into such scaffolds is approximately 80 μm. These results will direct the development of scaffolds with aligned macroarchitecture for tissue engineering bone.

Keywords
Bone tissue engineering; Hydroxyapatite; Scaffold; Macroarchitecture; Osteoblast
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In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 24, November 2004, Pages 5507–5514
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us