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A new method for isolation of polyethylene wear debris from tissue and synovial fluid

Paper ID Volume ID Publish Year Pages File Format Full-Text
12710 813 2004 7 PDF Available
Title
A new method for isolation of polyethylene wear debris from tissue and synovial fluid
Abstract

Sub-micron-sized ultrahigh molecular-weight polyethylene (PE) debris is generated in the joint space as a result of articulation and cyclic loading of an orthopaedic implant. Its characterization requires isolation and subsequent analysis by ultra-structural methods. An innovative method based on the digestion of paraffin-embedded tissue samples was proposed. Tissue slices were digested with sodium hypochlorite directly on polycarbonate filter. The same procedure could be applied also to fresh synovial fluid. Plastic particles were not lost or damaged during treatment. Chemical identification of particles was done by micro-Raman spectroscopy that confirmed purity of retrieved PE particles. Size and shape of PE particles were characterised using scanning electron microscopy and were comparable in number and morphology to the retrieval by other authors. Equivalent diameter ranged from 0.48 to 0.95 μm and particle number ranged from 9 to 23×109/cm3.

Keywords
Polyethylene; Wear debris; Scanning Electron Microscopy; Micro-Raman spectroscopy
First Page Preview
A new method for isolation of polyethylene wear debris from tissue and synovial fluid
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 24, November 2004, Pages 5531–5537
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering