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In vitro studies on the effect of delaminated a-C:H film fragments on bone marrow cell cultures ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
12728 814 2005 8 PDF Available
Title
In vitro studies on the effect of delaminated a-C:H film fragments on bone marrow cell cultures ☆
Abstract

Amorphous hydrogenated carbon (a-C:H) films have many outstanding properties required for a protective coating material on load bearing medical implants. Recently, titanium doped a-C:H films have been evaluated regarding their effects on bone marrow cell cultures. But many materials that are well-tolerated in bulk form are able to induce toxic reaction if present particulate form. In order to further assess biocompatibility aspects of these two coatings, film delamination has been mimicked in exposure to fluids. In the present study, particles from a-C:H, a-C:H/Ti and a-C:H–a-C:H/Ti bilayer films were added to bone marrow cell cultures in vitro. The results showed that plain a-C:H and to a certain extent a-CH/Ti particles were inert. Both kinds of particles did not significantly stimulate the osteoclast-related enzyme tartrate resistant acid phosphatase (TRAP). A slight increase in cell proliferation and total culture TRAP was found in cultures treated by a-C:H–a-C:H/Ti bilayer films. Latter effect can probably be traced back by the relative high percentage of small particles of a size of around 2 μm. However, if corrected by the cell number also no differences between particle-treated and untreated control cultures could be found, indicating the absence of a toxic effect from delaminated a-C:H coatings.

Keywords
ALP, alkaline phosphatase; RBMC, rat bone marrow cells; PBS, phosphate buffered saline; TRAP, tartrate-resistant acid phosphatase; a-C:H, amorphous hydrogenated carbonParticles; Bone marrow cells; a-C:H; Titanium; Biocompatibility
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In vitro studies on the effect of delaminated a-C:H film fragments on bone marrow cell cultures ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 17, June 2005, Pages 3487–3494
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us