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In vitro degradation of resin–dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
12776 816 2003 9 PDF Available
Title
In vitro degradation of resin–dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy
Abstract

Our knowledge of the mechanisms responsible for the degradation of resin–dentin bonds are poorly understood. This study investigated the degradation of resin–dentin bonds after 1 year immersion in water. Resin–dentin beams (adhesive area: 0.9 mm2) were made by bonding using a resin adhesive, to extracted human teeth. The experimental beams were stored in water for 1 year. Beams that had been stored in water for 24 h were used as controls. After water storage, the beams were subjected to microtensile bond testing. The dentin side of the fractured surface was observed using FE-SEM. Subsequently, these fractured beams were embedded in epoxy resin and examined by TEM. The bond strength of the control specimens (40.3±15.1 MPa) decreased significantly (p<0.01) after 1 year of water exposure (13.3±5.6 MPa). Loss of resin was observed within fractured hybrid layers in the 1 year specimens but not in the controls. Transmission electron microscopic examination revealed the presence of micromorphological alterations in the collagen fibrils after 1 year of water storage. These micromorphological changes (resin elution and alteration of the collagen fibrils) seem to be responsible for the bond degradation leading to bond strength reduction.

Keywords
Resin–tooth interface; Microtensile bond test; SEM; TEM; Collagen fibril; Hybrid layer
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In vitro degradation of resin–dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 21, September 2003, Pages 3795–3803
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us