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Human osteoblast response to pulsed laser deposited calcium phosphate coatings

Paper ID Volume ID Publish Year Pages File Format Full-Text
12805 818 2005 9 PDF Available
Title
Human osteoblast response to pulsed laser deposited calcium phosphate coatings
Abstract

Octacalcium phosphate (OCP) and Mn2+-doped carbonate hydroxyapatite (Mn-CHA) thin films were deposited on pure, highly polished and chemically etched Ti substrates with pulsed laser deposition. The coatings exhibit different composition, crystallinity and morphology that might affect their osteoconductivity. Human osteoblasts were cultured on the surfaces of OCP and Mn-CHA thin films, and the cell attachment, proliferation and differentiation were evaluated up to 21 days. The cells showed a normal morphology and a very good rate of proliferation and viability in every experimental time. Alkaline phosphatase activity was always higher than the control and Ti groups. From days 7 to 21 collagen type I production was higher in comparison with control and Ti groups. The level of transforming growth factor beta 1 (TGF-β1) was lower at 3 and 7 days, but reached the highest values during following experimental times (14 and 21 days). Our data demonstrate that both calcium phosphate coatings favour osteoblasts proliferation, activation of their metabolism and differentiation.

Keywords
Calcium phosphate coating; Octacalcium phosphate; Mn2+-doped carbonated hydroxyapatite; Laser ablation; Human osteoblasts; Differentiation
First Page Preview
Human osteoblast response to pulsed laser deposited calcium phosphate coatings
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 15, May 2005, Pages 2381–2389
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering