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An organic–inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
12817 818 2005 8 PDF Available
Title
An organic–inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor
Abstract

Much interest has recently been shown in the potential utility of bioartificial liver (BAL) as a bridge support for patients and as a module for experimental purposes. A radial-flow bioreactor (RFB), one of the perfused bed/scaffold-type bioreactors, enables a highly functional three-dimensional culture as BAL. The functional capacity of bioreactors depends not only on their mechanistic structures but also on scaffolds packed in them. In the present study, we examined the possible utility of a new porous organic–inorganic-hybrid scaffold in an RFB. The scaffold was made from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) by a sol–gel method using sieved sucrose particles as a porogen. In the porous TEOS–PDMS hybrid scaffold, human hepatocellular carcinoma cells (HepG2) proliferated actively and formed cell clusters more efficiently than they did in a polyvinyl-alcohol scaffold. When cultivated in PDMS–TEOS, HepG2 cells secreted a ∼three-fold greater amount of albumin than that secreted in a monolayer culture. For potential application of BAL to pharmacological studies and future clinical use, it is essential to develop a method to propagate liver cells that maintain highly specific functions. The present results indicate that PDMS–TEOS may be a promising scaffold for developing such functional culture methods.

Keywords
Three-dimensional culture; Organic–inorganic hybrid; Scaffold; Bioreactor; HepG2; TEOS–PDMS
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An organic–inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 15, May 2005, Pages 2509–2516
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us