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Synthesis of creatinine-imprinted poly(β-cyclodextrin) for the specific binding of creatinine

Paper ID Volume ID Publish Year Pages File Format Full-Text
12844 818 2005 8 PDF Available
Title
Synthesis of creatinine-imprinted poly(β-cyclodextrin) for the specific binding of creatinine
Abstract

An artificial receptor for creatinine was synthesized by the method of molecularly imprinted polymer (MIP). β-Cyclodextrin was used as a monomer cross-linked with epichlorohydrin in the presence of creatinine, which was a template for the imprinting. Different molar ratios of monomer to template were used to synthesize the polymers so that better specific adsorption ability towards creatinine could be achieved. The results showed that to carry out the polymerization with a molar ratio of monomer to template of 3:2 and monomer to cross-linking agent of 1:10 was proper. N-hydroxysuccinimide and 2-pyrrolinidone were used as the analogues of creatinine in the adsorption experiments of multi-component solutions to reveal the specific recognition ability of the molecularly imprinted poly(β-cyclodextrin) (poly(β-CD)) for the template molecule, creatinine. One such detection interference of creatinine was creatine, which also co-exists in serum. Hence, adsorption experiments of creatinine/creatine binary mixture were also carried out to investigate and confirm the specific binding of the creatinine-imprinted poly(β-cyclodextrin) towards creatinine. The hydroxyl groups of the imprinted poly(β-CD) was further capped by chlorotrimethylsilane (CTMS) to investigate the interaction between creatinine and the imprinted poly(β-CD). The adsorption resulting from the mixture solution by MIPs suggested that the creatinine-imprinted poly(β-CD) demonstrated superior binding effect for the target molecule, creatinine, rather than creatine, N-hydroxysuccinimide and 2-pyrrolinidone.

Keywords
Molecularly imprinted poly(β-cyclodextrin); Creatinine; N-hydroxysuccinimide; 2-pyrrolinidone; Creatine; Chlorotrimethylsilaneβ-CD, β-cyclodextrin; MIP, molecularly imprinted polymer; MAA, methacrylic acid; NVP, N-vinylpyridine; SM, styrene monomer; Non-M
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Synthesis of creatinine-imprinted poly(β-cyclodextrin) for the specific binding of creatinine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 15, May 2005, Pages 2759–2766
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us