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Glass reinforced hydroxyapatite for hard tissue surgery—Part II: in vitro evaluation of bone cell growth and function

Paper ID Volume ID Publish Year Pages File Format Full-Text
12874 820 2001 8 PDF Available
Title
Glass reinforced hydroxyapatite for hard tissue surgery—Part II: in vitro evaluation of bone cell growth and function
Abstract

Hydroxyapatite (HA)-based materials are considered to be potentially useful as bone implant materials, particularly those reinforced with glass to improve mechanical strength. However, the precise effects of glass-reinforced HA on the growth and functions of bone cells are still unclear. The present study has therefore examined the response of human osteoblast-like cells to HA and HA reinforced with two different proportions of glass, namely 2.5% and 5%.All materials enabled the cells to attach and proliferate during 7 days in culture and, although the growth was less than on control plastic surfaces, there was no deleterious effect of the 5% glass composite compared with HA alone. Flow cytometry analysis showed that there was no effect on cell size and granularity, but there were marked and highly selective changes in the expression of certain connective tissue proteins. Thus, while bone sialoprotein and osteonectin were down-regulated on HA alone, the expression of these antigens was relatively enhanced on the composite materials, and collagen type I was also up-regulated on the glass-reinforced HA. Thus, modulation of the glass composition of HA materials could be used to produce not only improved mechanical strength, but also enhanced biocompatibility.

Keywords
Glass-reinforced HA; Bone cells; Growth; Flow cytometry
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Glass reinforced hydroxyapatite for hard tissue surgery—Part II: in vitro evaluation of bone cell growth and function
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 20, 15 October 2001, Pages 2817–2824
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us