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A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
12902 822 2004 9 PDF Available
Title
A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis
Abstract

In this study, L929 cell adhesion, proliferation and apoptosis were investigated on chitosan (CS) and chitosan–gelatin (CS-Gel) membranes having different degrees of deacetylation (DD). The mitochondrial activity [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay results demonstrated that the DD played a key role in the L929 cell adhesion. Indeed, the higher the DD of a CS membrane, the stronger the cell adhesion. The cell cycle analysis showed that the CS surface inhibits most of the cells entering the cell cycle and the DD of CS had little effect on the cell cycle progression. On the other hand, this study also confirmed that CS, blended with Gel, induced L929 cells to enter the cell cycle, encouraged proliferation, decreased the degree of apoptosis on the CS membranes, and are comparable to the results from L929 cells on tissue culture plates. In brief, CS modified with Gel improves cytocompatibility by shielding the positively charged CS with a high charge density.

Keywords
Chitosan; Gelatin; Cell cycle analysis; Apoptosis; Cytocompatibility
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A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 18, August 2004, Pages 3973–3981
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us