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Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
12910 822 2004 10 PDF Available
Title
Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells
Abstract

In this work, poly l-lactide (PLLA) membranes with different morphologies were prepared and the equilibrium phase diagram of membrane formation system of ethanol, methylene chloride, and PLLA was studied. Based on the phase diagram, particulate and porous membranes, dominated by crystallization and liquid–liquid demixing, respectively, could be prepared by changing the PLLA concentration of casting solution. In addition, in vitro interaction of MG-63 osteosarcoma cells and PLLA membranes with dense, porous and particulate morphologies was investigated. It was found that the particulate membrane not only could improve cell adhesion and growth, but also could upregulate the osteoblastic phenotype. Therefore, the PLLA membrane with particulate morphology satisfies the biomaterial requirement necessary for temporary scaffold to transplanted osteoblasts and provides a means for the architectural design of more complex tissue-engineered systems.

Keywords
PLLA; Particulate membranes; MG-63 cells
First Page Preview
Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 18, August 2004, Pages 4047–4056
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering