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Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives

Paper ID Volume ID Publish Year Pages File Format Full-Text
12927 822 2004 8 PDF Available
Title
Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives
Abstract

Photosensitive poly(allylamine) containing β-galactose moieties in the side chain (LPAN3) was prepared by the reaction of poly(allylamine) with lactobionic acid and azidobenzoic acid. To create micropatterned surfaces, a LPAN3-coated poly(methyl methacrylate) (PMMA) substrate was irradiated with an ultraviolet lamp under a photomask. The presence of a LPAN3 layer on the substrate was confirmed using an electron spectroscopy for chemical analysis. Micropatterned cell culture was carried out by seeding hepatocytes and/or fibroblasts on the substrate. As a result, hepatocytes and fibroblasts adhered only to the LPAN3 and PMMA lane, respectively. Co-culture on the stripe patterned substrate was carried out by the first cell seeding of hepatocytes and subsequently by the second cell seeding of fibroblasts. The co-cultured cells produced extracellular matrix such as fibronectin, suggesting a biological function.

Keywords
Poly(allylamine); Micropatterned surface; Hepatocyte; Co-culture
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Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 18, August 2004, Pages 4225–4232
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us