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Novel approach to fabricate keratin sponge scaffolds with controlled pore size and porosity

Paper ID Volume ID Publish Year Pages File Format Full-Text
12930 822 2004 8 PDF Available
Title
Novel approach to fabricate keratin sponge scaffolds with controlled pore size and porosity
Abstract

A compression-molding/particulate-leaching (CM/PL) method was developed to fabricate S-sulfo keratin sponges with the controlled pore size and porosity. The S-sulfo keratin was extracted from wool and was then spray-dried to give S-sulfo keratin powder. The S-sulfo keratin powder mixed with urea in advance was compression-molded together with the sieved NaCl particulates above the melting temperature of urea. The following removal of the salts and urea in water created the sponges composed of interconnected pores and the continuous S-sulfo keratin matrix. The S-sulfo keratin sponges were strong enough to handle and water-insoluble. By contrast, the sponges prepared without urea were very fragile and readily collapsed, because most of S-sulfo keratin matrix remained powdery. The pore size was in good accordance with the size of the salts, indicating that the pores were formed by leaching-out the salts. The S-sulfo keratin sponges with the regulated sizes of pores (<100, 100–300 and 300–500 μm) were fabricated, all of which had more than 90% of the porosity. Thus, CM/PL method is able to give the S-sulfo keratin sponge with the desired pore size and porosity, which might be a good scaffold for the cells in tissue engineering.

Keywords
Keratin; Compression-molding; Particulate-leaching; Sponge; Scaffold
First Page Preview
Novel approach to fabricate keratin sponge scaffolds with controlled pore size and porosity
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 18, August 2004, Pages 4255–4262
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering