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Biological glass coating on ceramic materials:: in vitro evaluation using primary osteoblast cultures from healthy and osteopenic rat bone

Paper ID Volume ID Publish Year Pages File Format Full-Text
12971 823 2001 9 PDF Available
Title
Biological glass coating on ceramic materials:: in vitro evaluation using primary osteoblast cultures from healthy and osteopenic rat bone
Abstract

ZrO2 and Al2O3 substrates were successfully coated by a double layer of a silica-based glass named RKKP, using a low-cost firing technique. RKKP is a glass well known for its bioactivity; therefore, a RKKP coating on Al2O3 or ZrO2, allows to combine the excellent mechanical properties of these strong ceramic substrates with its bioactivity. ZrO2 samples were easily coated using a double layer of RKKP by a simple enamelling technique. To accommodate the thermal expansion coefficient mismatch between Al2O3 and RKKP, this substrate was coated using a multilayered composite approach. All of the coatings were characterised from a morphological and compositional point of view, and an extensive biological evaluation was performed using fresh rat osteoblasts. Osteoblast primary cultures were derived from the trabecular bone of femoral condyles harvested from intact (NB) and osteopenic (OB) rats. After characterisation of their phenotype, osteoblasts were seeded on material samples of ZrO2 or Al2O3 coated with RKKP, and cultured for 7 days. Cell proliferation (MTT test) and cell differentiation (alkaline phosphatase activity) were evaluated at the end of the experiment, to assess osteoblast behaviour in the presence of biomaterials and determine if the results were related to the host bone quality. Results of both materials showed a good level of biocompatibility. In particular, MTT significant higher values were detected in NB cultures on ZrO2–RKKP samples; ALP activity significantly increased in NB cultures on Al2O3–RKKP and in OB cultures on both coated samples.

Keywords
Biomaterials; Bone; Cell cultures; Osteopenia
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Biological glass coating on ceramic materials:: in vitro evaluation using primary osteoblast cultures from healthy and osteopenic rat bone
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 18, September 2001, Pages 2535–2543
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us