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Protein characterisation of salivary and plasma biofilms formed in vitro on non-corroded and corroded dental ceramic materials

Paper ID Volume ID Publish Year Pages File Format Full-Text
12972 823 2001 11 PDF Available
Title
Protein characterisation of salivary and plasma biofilms formed in vitro on non-corroded and corroded dental ceramic materials
Abstract

Dental ceramics are generally regarded as low-adhesive materials. Different ceramics may, however, differ in composition and physico-chemical surface properties, which may be changed after corrosion. The aim of this study was to examine the adsorption of proteins onto specimens of different ceramic materials after the incubation in saliva and plasma before and after in vitro corrosion. In addition, the topography of the biofilm was examined by AFM. Surface-bound proteins were desorbed and analysed by polyacrylamide gel electrophoresis (PAGE) and immunoblotting using antibodies to saliva and plasma proteins. Silver-stained gels indicated differences in the adsorption of proteins. Differences in surface roughness at the nanometer level did not, however, seem to be correlated to the protein adsorption. After corrosion, unchanged or increased protein staining was generally seen in the gels and Western blots. The reactions for salivary amylase and proline-rich proteins varied between the different materials. Albumin and fibrinogen were identified in samples from all materials tested. Fibronectin and in specific IgA were more sparsely seen. No saliva but all plasma proteins were identified in the alumina and yttria-stabilised zirconia samples and reduced protein reactions were obtained after corrosion.

Keywords
Dental ceramics; Biofilm; Corrosion; Electrophoresis; Saliva; Plasma; Biomaterials
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Protein characterisation of salivary and plasma biofilms formed in vitro on non-corroded and corroded dental ceramic materials
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 18, September 2001, Pages 2545–2555
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us