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UHMWPE oxidation increases granulocytes activation: a role in tissue response after prosthesis implant

Paper ID Volume ID Publish Year Pages File Format Full-Text
12986 826 2003 6 PDF Available
Title
UHMWPE oxidation increases granulocytes activation: a role in tissue response after prosthesis implant
Abstract

Ultra-high molecular weight polyethylene (UHMWPE), a biopolymer widely used in orthopaedic implants, is oxidized during γ-ray sterilization; such surface oxidation is considered as major responsible for inflammation and prosthesis failure. As granulocytes are involved in first contact inflammation, we have measured their oxidative burst by flow cytometry using dihydrorhodamine 123 (DRH) to evaluate their activation following contact with normal and oxidized UHMWPE. Peripheral blood cells (obtained by lysed blood) were loaded with DRH, seeded onto polystyrene, normal and heat-oxidized UHMWPE disks for 30 min and then collected for analysis. Granulocytes were individuated using FSC and SSC signals and their cell associated green fluorescence was analyzed. Both normal and oxidized UHMWPE stimulated granulocytes activation as showed by the mean fluorescence emitted (109.3±3.8 and 150.1±9.2, respectively) compared to control samples (81.6±0.3). Moreover oxidized UHMWPE activated a significantly higher percentage of granulocytes (73.35±5.2%) compared to not-oxidized UHMWPE (21.5±3.8%). UHMWPE surface oxidation responsible for increased granulocyte activation seems to play a role in tissue response to implants.

Keywords
Biocompatibility; Granulocytes activation; Dihydrorhodamine 123; UHMWPE; Flow cytometry; Surface oxidation; Sterilization
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UHMWPE oxidation increases granulocytes activation: a role in tissue response after prosthesis implant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 17, August 2003, Pages 2895–2900
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us