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TAK-778 enhances osteoblast differentiation of human bone marrow cells cultured on titanium

Paper ID Volume ID Publish Year Pages File Format Full-Text
12990 826 2003 6 PDF Available
Title
TAK-778 enhances osteoblast differentiation of human bone marrow cells cultured on titanium
Abstract

TAK-778 induces bone growth in in vitro and in vivo models. The aim of this study was to evaluate the osteogenic potential of TAK-778 on human bone marrow cells cultured on commercially pure titanium (cpTi). Cells were cultured either in absence or in presence of TAK-778 (10−5 m) on cpTi in supplemented α-MEM. For attachment evaluation, cells were cultured for 4 and 24 h. After 7, 14, and 21 days, cell proliferation, cell viability, total protein content, alkaline phosphatase (ALP) activity, and bone-like formation were evaluated. TAK-778 did not affect cell attachment and viability. Cell number was reduced by TAK-778. ALP activity, total protein content, and bone-like formation were increased by TAK-778. These results suggest that initial cell events such as cell attachment are not affected by TAK-778 while events that indicate osteoblast differentiation including reduced cell proliferation, and increased both ALP activity and bone-like formation are enhanced by TAK-778 in presence of cpTi. It means that TAK-778 could be a useful drug to improve the osseointegration of implants by both enhancing and accelerating bone formation on Ti surface.

Keywords
Differentiation; Human bone marrow cells; Osteoblast; TAK-778; Titanium
First Page Preview
TAK-778 enhances osteoblast differentiation of human bone marrow cells cultured on titanium
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 17, August 2003, Pages 2927–2932
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering