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Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
13000 827 2001 11 PDF Available
Title
Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold
Abstract

A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

Keywords
Hyaluronic acid polymer; Mineralization; Stromal cells; Bone matrix protein; Calcium; Alkaline phosphatase
First Page Preview
Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 15, 1 August 2001, Pages 2095–2105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering