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Surface-adsorbed α1-microglobulin modulation of human fibroblasts spreading and matrix metalloproteinases release

Paper ID Volume ID Publish Year Pages File Format Full-Text
13021 828 2004 5 PDF Available
Title
Surface-adsorbed α1-microglobulin modulation of human fibroblasts spreading and matrix metalloproteinases release
Abstract

The lipocalin α1-microglobulin (α1-m), an immunoregulatory protein produced by human hepatocytes and distributed in various organs and fluids, is physiologically adsorbed onto polymer surfaces from both serum and urine, and its adsorption correlated to the degree of surface hydrophobicity. Starting from the hypothesis that α1-m holds a modulatory role at the biomaterials–tissue interface, we have observed a dose-dependent reduction in adhesion of human fibroblasts (cell line MRC-5) seeded onto polystyrene (PS) in a serum-free medium in the presence of adsorbed α1-m (2.1±0.2×104 cells/cm2 at 200 ng/ml α1-m ) compared to cells seeded onto cell grade PS (2.9±0.05×104 cells/cm2) after 72 h. Moreover, in the presence of α1-m, adherent MRC-5 cells exhibit an altered shape due to inhibition of cell spreading, and release of matrix metalloproteinase -2 (gelatinase A, MMP-2) by fibroblasts was also increased by 1.6–1.9-fold after 72 h of incubation. These data extend the known spectrum of α1-m activities, suggesting a possible role of this protein in the complex series of events occurring at the tissue–biomaterial interface.

Keywords
α1-microglobulin; Protein adsorption; Cell adhesion; Spreading; MMP-2; Fibroblast
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Surface-adsorbed α1-microglobulin modulation of human fibroblasts spreading and matrix metalloproteinases release
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 17, August 2004, Pages 3439–3443
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us