fulltext.study @t Gmail

Layer-by-layer deposition of hyaluronic acid and poly-l-lysine for patterned cell co-cultures ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
13036 828 2004 10 PDF Available
Title
Layer-by-layer deposition of hyaluronic acid and poly-l-lysine for patterned cell co-cultures ☆
Abstract

A novel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Non-biofouling hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-l-lysine (PLL) to HA patterns was used to switch the HA surfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications.

Keywords
Patterned co-cultures; Layer-by-layer; Hyaluronic acid; Poly-l-lysine
First Page Preview
Layer-by-layer deposition of hyaluronic acid and poly-l-lysine for patterned cell co-cultures ☆
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 17, August 2004, Pages 3583–3592
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us