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Denatured collagen as support for a FGF-2 delivery system: physicochemical characterizations and in vitro release kinetics and bioactivity

Paper ID Volume ID Publish Year Pages File Format Full-Text
13056 828 2004 12 PDF Available
Title
Denatured collagen as support for a FGF-2 delivery system: physicochemical characterizations and in vitro release kinetics and bioactivity
Abstract

Collagen-based materials have scaffold properties to support bioactive molecules such as growth factor (GF). Gelatin, a denatured collagen, may have also some potential to interact with GF. An alternative process to denature collagen using trifluoroacetic acid (TFA) was investigated. Physicochemical characterization (XPS, DSC, isoelectric point, water uptake) of TFA-denatured collagen was comparable to regular gelatin, except a significant hydrophilicity and a pH sensitivity. FGF-2 was mixed with either regular gelatin or TFA-denatured collagen, then incorporated to a collagen sponge. Autoradiography revealed a relatively homogenous distribution of radiolabeled FGF-2 within the sponge. In vitro release kinetic of radiolabeled FGF-2 was investigated as well as the bioactivity of FGF-2 towards endothelial cell growth. The mixture was also sorbed to hydrogels made of ethylene vinyl acetate co-polymer and poly(2-hydroxyethyl methacrylate), and to cell culture insert membranes as control. Release of FGF-2 from collagen was progressive in the presence of TFA-denatured collagen, and cell growth was stimulated (significant peak at 8 and 10 days) by TFA-denatured collagen and FGF-2 eluted particularly from collagen sponges. Whereas control hydrogels, and those with regular gelatin showed a early stimulation of cell growth (1–5 days). Thus, the combination of both FGF-2 and an acid-denatured collagen in collagen sponges allows to sustain in vitro endothelial cell activity.

Keywords
Collagen; Growth factors; Gelatin; Bioactivity; In vitro assay; Growth factor release
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Denatured collagen as support for a FGF-2 delivery system: physicochemical characterizations and in vitro release kinetics and bioactivity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 17, August 2004, Pages 3761–3772
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us