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A novel porous cells scaffold made of polylactide–dextran blend by combining phase-separation and particle-leaching techniques

Paper ID Volume ID Publish Year Pages File Format Full-Text
13081 829 2002 10 PDF Available
Title
A novel porous cells scaffold made of polylactide–dextran blend by combining phase-separation and particle-leaching techniques
Abstract

In this study, a kind of biodegradable material was developed by blending polylactide (PLA) with natural biodegradable dextran, and a novel sponge-like scaffold made of it was fabricated thereof using solvent-casting and particle-leaching technique. To obtain a uniform blend of PLA and dextran by simple solvent-casting method, hydroxyls of dextran should be protected via trimethylsilyl (TMS) groups to make dextran soluble in organic solvents. Benzene was found among the few solvents that could dissolve this TMS-protected dextran (TMSD) well, however, it was not a good solvent for PLA. Therefore, a homogeneous mixed solution of PLA and TMSD could be obtained when a mixture of dichloroform (DCM) and benzene (v/v=6/4) was used. By this technique, PLA–dextran blend films and even PLA films were observed a microporous structure (pore size around 5–10 μm) formation throughout the films under scanning electron microscope (SEM). Scaffolds that were prepared by dissolving PLA and TMSD in mixed solvent of DCM and benzene and using salt as porogen, were observed the formation of micropores (pore size around 5–10 μm) in the cellular walls of macropores (pore size around 100–200 μm). This microporous structure was closely related to the phase separation occurring during films or foams formation, which was mainly due to the different solubility of PLA and TMSD in benzene, as well as the different evaporation rates of DCM and benzene. In comparison with PLA, the surface and bulk hydrophilicity of PLA–dextran blend films or foams were significantly improved after the TMS groups were removed in methanol, and the results of cell culture on these polymeric substrates exhibited an enhancement on cell attachment and proliferation.

Keywords
Polylactide; Dextran; Cells scaffold; Phase separation; Particle leaching
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A novel porous cells scaffold made of polylactide–dextran blend by combining phase-separation and particle-leaching techniques
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 23, December 2002, Pages 4483–4492
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us