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Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127

Paper ID Volume ID Publish Year Pages File Format Full-Text
13096 829 2002 5 PDF Available
Title
Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127
Abstract

We investigate the gelation of a poly(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide) copolymer, Pluronic® F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol–gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics® and applications where copolymer concentration must be minimized due to toxicity concerns.

Keywords
Sol–gel boundary; Cell culture; Encapsulation; PEO–PPO–PEO; Triblock copolymers
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Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 23, December 2002, Pages 4615–4619
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us