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Microtextured substrata alter gene expression, protein localization and the shape of cardiac myocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
13106 831 2003 14 PDF Available
Title
Microtextured substrata alter gene expression, protein localization and the shape of cardiac myocytes
Abstract

Many of the experiments designed to understand fundamental principles in cardiac physiology are performed in vitro using myocytes isolated from adult or neonatal hearts. However, these cells have probably lost some of their original properties in culture prior to study. Our objective is to recapitulate cardiac myocyte structure and function by growing cells on microtextured silicone substrata produced by photolithography and microfabrication techniques. Myocytes are plated on nontextured, micropegged (5 μm high), microgrooved (parallel grooves with a depth of 5 μm) or combination (micropegged and grooved) substrata. Myocytes plated on microtextured surfaces display a change in cell shape with an increase in myofibrillar height and a decrease in cell area. This shape change did not affect the stoichiometry of the myofibrillar proteins but did elicit microenvironmental remodeling of proteins that mechanically attach the cell to its surroundings. Cells terminate in a sarcomeric striation on the vertical interface of the peg whereas on nontextured surfaces they end in long nonstriated cables. Vinculin, a focal adhesion protein, was found to decrease in expression on combination surfaces as compared to nontextured substrata. A three-dimensional microtextured substratum appears to reintroduce a more physiological microarchitecture for tissue culture that may have potential uses in biological research as well as in tissue engineering and diagnostic applications.

Keywords
Microtopography; Three-dimensional; Cardiomyocyte; Cell attachment
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Microtextured substrata alter gene expression, protein localization and the shape of cardiac myocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 14, June 2003, Pages 2463–2476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us