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Adipose tissue engineering based on human preadipocytes combined with gelatin microspheres containing basic fibroblast growth factor

Paper ID Volume ID Publish Year Pages File Format Full-Text
13111 831 2003 9 PDF Available
Title
Adipose tissue engineering based on human preadipocytes combined with gelatin microspheres containing basic fibroblast growth factor
Abstract

Gelatin microspheres containing basic fibroblast growth factor (bFGF) were prepared for the controlled release of bFGF. Co-implantation with the gelatin microspheres enabled preadipocytes to induce adipose tissue formation at the implanted site. Preadipocytes isolated from human fat tissue were suspended with the gelatin microspheres containing bFGF and incorporated into a collagen sponge of cell scaffold. Following subcutaneous implantation of the collagen sponge incorporating human preadipocytes, and gelatin microspheres containing 1 μg of bFGF into the back of nude mice, adipose tissue was formed at the implanted site of collagen sponge within 6 weeks postoperatively although the extent depended on the number of preadipocytes transplanted and the bFGF dose. The formation of adipose tissue was significant compared with the implantation of collagen sponge incorporating human preadipocytes and 1 μg of free bFGF. The area of adipose tissue newly formed was increased with the number of preadipocytes transplanted until to 1.0×105 cells/site and thereafter leveled off. The maximum area was observed at the bFGF dose of 1 μg/site. The area was significantly smaller at the bFGF dose of 0.5 μg/site or larger than 1 μg/site. Immunohistochemical examination indicated that the adipose tissue newly formed was composed of human matured adipocytes. No adipogenesis was observed at the implanted site of collagen sponge incorporating either gelatin microspheres containing bFGF or human preadipocytes and the mixed gelatin microspheres containing bFGF and human preadipocytes. We conclude that combination of gelatin microspheres containing bFGF and preadipocytes with the collagen sponge is essential to achieve tissue engineering of fat tissue.

Keywords
Adipose tissue engineering; Human preadipocytes; bFGF release; Gelatin microspheres; A collagen sponge
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Adipose tissue engineering based on human preadipocytes combined with gelatin microspheres containing basic fibroblast growth factor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 14, June 2003, Pages 2513–2521
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us