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Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials

Paper ID Volume ID Publish Year Pages File Format Full-Text
13120 832 2011 9 PDF Available
Title
Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials
Abstract

Adhesion of bacteria at the surface of implanted materials is the first step in microbial infection, leading to post-surgical complications. In order to reduce this adhesion, we show that poly(l-lysine)/poly(l-glutamic acid) (PLL/PGA) multilayers ending by several PLL/PGA-g-PEG bilayers can be used, PGA-g-PEG corresponding to PGA grafted by poly(ethylene glycol). Streaming potential and quartz crystal microbalance-dissipation measurements were used to characterize the buildup of these films. The multilayer films terminated by PGA and PGA-g-PEG were found to adsorb an extremely small amount of serum proteins as compared to a bare silica surface but the PGA ending films do not reduce bacterial adhesion. On the other hand, the adhesion of Escherichia coli bacteria is reduced by 72% on films ending by one (PLL/PGA-g-PEG) bilayer and by 92% for films ending by three (PLL/PGA-g-PEG) bilayers compared to bare substrate. Thus, our results show the ability of PGA-g-PEG to be inserted into multilayer films and to drastically reduce both protein adsorption and bacterial adhesion. This kind of anti-adhesive films represents a new and very simple method to coat any type of biomaterials for protection against bacterial adhesion and therefore limiting its pathological consequences.

Keywords
Polyelectrolyte multilayers; Poly(ethylene glycol); Protein adsorption; Bacterial adhesion; Surface modification
First Page Preview
Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 11, May 2004, Pages 2003–2011
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering