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Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
13128 832 2004 5 PDF Available
Title
Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts ☆
Abstract

The aim of this study was to determine chitosan's effect on osteoblast and fibroblast cell attachment. Mouse MC3T3-E1 osteoblasts and 3T3 fibroblasts were grown in the presence of serum on two commercially available chitosans, Chitosan-H (CH) and Protasan CL212 (PR). Cell attachment and immunofluorescent analysis at various time points were done to analyze initial phenotypic profiles. At 1 h, significantly (P<0.05) fewer fibroblasts attached to CH or PR than serum-coated substrates. Osteoblast attachment to the same biopolymers at 1 h was significantly greater than those seen with fibroblasts. At 24 h, levels of cell attachment for fibroblasts to both CH and PR significantly increased and were similar to levels seen in osteoblast cultures at both 1 and 24 h. Morphologically, immunofluorescent analysis showed that osteoblasts plated on the biopolymers were attached and beginning to spread at 1 h, whereas fibroblasts appeared more rounded. At 24 h, fibroblasts plated on CH or PR revealed a heterogeneous population of round and semi-spread cells. In comparison, osteoblasts displayed phenotypes that were well spread with a developed cytoskeleton. These results suggest that CH and PR support the initial attachment and spreading of osteoblasts preferentially over fibroblasts, and that manipulation of the biopolymer can alter the level of attachment and spreading.

Keywords
Cell culture; Fibroblasts; Osteoblasts; Drug delivery; Chitosan
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Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 11, May 2004, Pages 2075–2079
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us