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In vitro and in vivo evaluation of alginate/sol–gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas

Paper ID Volume ID Publish Year Pages File Format Full-Text
13145 834 2002 7 PDF Available
Title
In vitro and in vivo evaluation of alginate/sol–gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas
Abstract

Alginate/aminopropyl-silicate/alginate (Alg/AS/Alg) membrane was prepared on Ca-alginate gel beads by a sol–gel process. The membrane has ≡Si–O–Si≡ bonds as well as electrostatic bonds between amino groups of AS and carboxyl groups of alginate. Permeability and stability were investigated for the membrane. Furthermore, rat islets encapsulated in the membrane (499±32 μm in diameter, 1000 islets/recipient) were transplanted to the peritoneal cavities of the mice with streptozotocin-induced diabetes. Our data show that the membrane had the molecular weight cut-off point of between 70 and 150 kDa, and hardly inhibited the permeation of glucose and insulin. The Alg/AS/Alg microcapsule was more stable than the well-known Alg/poly-l-lysine (PLL)/Alg microcapsule. After 30 days of soaking in stimulated body fluid, the percentages of intact microcapsule were 98.4±0.5 (mean±SEM)% and 88.0±1.5% (p<0.001) for the Alg/AS/Alg and Alg/PLL/Alg microcapsules, respectively. The maximum maintenance period of normoglycemia was 105 days without administration of immunosuppressive drugs.

Keywords
Sol–gel process; Immunoisolation membrane; Microcapsule; Islet; Aminopropyl-silicate
First Page Preview
In vitro and in vivo evaluation of alginate/sol–gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 21, November 2002, Pages 4177–4183
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering