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Development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs

Paper ID Volume ID Publish Year Pages File Format Full-Text
13187 840 2004 11 PDF Available
Title
Development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs
Abstract

This study investigates the utility of genetically modified cells developed for the qualitative and quantitative non-destructive evaluation of cells on biomaterials. The Fisher rat fibroblastic cell line has been genetically modified to stably express the reporter genes enhanced green fluorescence protein (EGFP) and luciferase. These reporter genes provide two unique opportunities to evaluate cell growth on materials without destruction of the sample. Utilizing the fluorescence of EGFP expressed in the cells, we were able to demonstrate distribution of cells in a oligo(poly(ethylene glycol) fumarate) hydrogel material and on a titanium fiber mesh scaffold using an inverted fluorescent light microscope. In addition, we were able to utilize a molecular light imaging system to macroscopically image the cells on these materials both with fluorescence and luminescence, as well as quantify the signal from the samples. Quantification of cell growth on the titanium mesh material for a period of 28 days was accomplished using the molecular light imaging system. Imaging was extended in vivo to cells on the titanium mesh scaffolds subcutaneously implanted in Fisher rats for a period of 28 days. This study outlines a non-destructive method to evaluate cells growing on biomaterials in vitro and in vivo.

Keywords
Tissue engineering; Genetically modified cells; Imaging; Reporter genes
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Development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 27, December 2004, Pages 5809–5819
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us