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In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody: potential use for local anti-cytokine antibody therapy on surgically resected tissues

Paper ID Volume ID Publish Year Pages File Format Full-Text
13193 840 2004 7 PDF Available
Title
In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody: potential use for local anti-cytokine antibody therapy on surgically resected tissues
Abstract

Styrenated antibody (ST-Ab) and styrenated gelatin (ST-gelatin) were prepared by condensation reaction of antibody or gelatin with 4-vinylbenzoic acid, respectively. The affinity loss of ST-Ab to its antigen was minimal. ST-Ab and ST-gelatin were copolymerized with by visible-light irradiation in the presence of a water-soluble camphorquinone as a photoinitiator to produce a tissue-adhesive, in situ-formed co-gel of ST-gelatin and ST-Ab. The amount of non-reacted ST-Ab released from the co-gel of ST-gelatin and ST-Ab into the medium was minimal. The confocal laser scanning microscopy observation showed that local accumulation of rhodamine-labeled bovine serum albumin (BSA) as a model antigen was noticed in the surface-to-subsurface region of the co-gel of ST-gelatin and anti-BSA ST-Ab, indicating that the gel prevented the permeation of BSA into the gel. In invasion double chamber assay using anti-hepatocyte growth factor (HGF) antibody, the co-gel prevented HGF-dependent invasion of pancreatic cancer cells. The discussion was made for potential application of an in situ-formed tissue-adhesive co-gel of ST-gelatin and ST-Ab, developed in this study, as a cytokine-barrier on a surgically resected tissue where cancer cells might still remain after resection of cancerous tissue.

Keywords
Photoreactive gelatin; Photoreactive antibody; Local antibody therapy
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In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody: potential use for local anti-cytokine antibody therapy on surgically resected tissues
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 27, December 2004, Pages 5867–5873
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us