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Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
13230 845 2002 6 PDF Available
Title
Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts
Abstract

Microcontact printing techniques were used to pattern circles (diameters 10, 50, 100, and 200 μm) of N1[3-(trimethoxysilyl)propyl]diethylenetriamine (DETA) surrounded by octadecyltrichlorosilane (OTS) borders on borosilicate glass, a model substrate. The DETA regions were further modified by immobilization of either the cell-adhesive peptides Arginine–Glycine–Aspartic Acid–Serine (RGDS) and Lysine–Arginine–Serine–Arginine (KRSR) or the non-adhesive peptides Arginine–Aspartic Acid–Glycine–Serine (RDGS) and Lysine–Serine–Serine–Arginine (KSSR). After four hours under standard cell culture conditions but in the absence of serum, adhesion of either osteoblasts or fibroblasts on surfaces patterned with the non-adhesive peptides RDGS and KSSR was random and low. In contrast, both osteoblasts and fibroblasts adhered and formed clusters onto circles modified with the adhesive peptide RGDS, whereas only osteoblasts adhered and formed clusters onto the circles modified with KRSR, a peptide that selectively promotes adhesion of osteoblasts. These results provide evidence that patterning of select peptides can direct adhesion of specific cell lines exclusively to predetermined regions on material surfaces.

Keywords
Micropatterning; Peptides; Cell adhesion; Osteoblasts; Fibroblasts
First Page Preview
Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 19, October 2002, Pages 3937–3942
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering