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Vascular cell responses to polysaccharide materials:: in vitro and in vivo evaluations

Paper ID Volume ID Publish Year Pages File Format Full-Text
13273 847 2000 8 PDF Available
Title
Vascular cell responses to polysaccharide materials:: in vitro and in vivo evaluations
Abstract

Chitosan has shown promise as a structural material for a number of tissue engineering applications. Similarly the glycosaminoglycans (GAGs) and their analogs have been known to exert a variety of biological activities. In this study we evaluated the potential of GAG–chitosan and dextran sulfate (DS)–chitosan complex materials for controlling the proliferation of vascular endothelial (EC) and smooth muscle cells (SMC). GAG–chitosan complex membranes were generated in vitro and seeded with human ECs or SMCs for culture up to 9 d. In addition, porous chitosan and GAG–chitosan complex scaffolds were implanted subcutaneously in rats to evaluate the in vivo response to these materials. The results indicated that while chitosan alone supported cell attachment and growth, GAG–chitosan materials inhibited spreading and proliferation of ECs and SMCs in vitro. In contrast, DS–chitosan surfaces supported proliferation of both cell types. In vivo, heparin–chitosan and DS–chitosan scaffolds stimulated cell proliferation and the formation of a thick layer of dense granulation tissue. In the case of heparin scaffolds the granulation layer was highly vascularized. These results indicate that the GAG–chitosan materials can be used to modulate the proliferation of vascular cells both in vitro and in vivo.

Keywords
Chitosan; Glycosaminoglycans; Heparin; Dextran sulfate; Endothelial cells; Smooth muscle; Scaffold; Tissue response
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Vascular cell responses to polysaccharide materials:: in vitro and in vivo evaluations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 22, 15 November 2000, Pages 2315–2322
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us