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Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application

Paper ID Volume ID Publish Year Pages File Format Full-Text
1329 81 2009 7 PDF Available
Title
Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application
Abstract

Microparticulates are useful for directly filling defective tissues as well as for delivering cells and bioactive molecules in regenerative medicine. This paper reports on the production of bioactive ceramic microspheres with an interconnected macropore structure. The sol–gel derived calcium silicate powder was homogenized with an oligomeric Camphene melt, which was used as a novel porogen, and spherical-shaped microparticulates were obtained by an oil-in-water emulsion method. A porous structure was generated through the sublimation of Camphene within the calcium silicate–Camphene solidified blend under ambient conditions. The microspheres retained the crystalline phase of apatite and wollastonite during heat treatment and induced calcium phosphate precipitation under a body-simulating medium, showing the characteristics of bone-bioactive materials. Osteoblastic cells were observed to anchor to and spread well over the surface of the porous microspheres, and further to proliferate actively with culturing time. The bioactive and porous microspheres developed are considered potentially useful in the regeneration of hard tissues as a matrix for tissue engineering as well as a direct filling material.

Keywords
Bioactive ceramics; Microspheres; Porous structure; Osteoblast culturing; Tissue engineering
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Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 5, June 2009, Pages 1725–1731
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us