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Molecular weight and degree of deacetylation effects on lipase-loaded chitosan bead characteristics

Paper ID Volume ID Publish Year Pages File Format Full-Text
13305 849 2002 8 PDF Available
Title
Molecular weight and degree of deacetylation effects on lipase-loaded chitosan bead characteristics
Abstract

The effects of the molecular weight (MW) and degree of deacetylation (DD) of chitosan on chitosan hydrogel beads were characterized, and the entrapment efficiency, release of entrapped lipase, and activity of immobilized Candida rugosa lipase were investigated. Fresh and freeze-dried beads were characterized. A solution of lipase was prepared in a 1.5% (w/v) chitosan and 1% (v/v) acetic acid medium, and then dropped into a tripolyphosphate solution to prepare the beads. The release studies were performed over 36 h. The enzyme activity was assayed using the Sigma lipase activity method. Chitosan with high MW and DD resulted in a higher loading. A lower activity was observed for beads produced with high DD chitosan. MW did not have a marked effect on the activity. The release study revealed that enzyme release increased to a maximum when the bead was manufactured with a low MW and a moderate to high DD chitosan sample. Freeze drying did not affect the release or the activity of the lipase. Chitosan with a high MW and DD can thus improve loading and reduce the release of lipase in these beads. The choice of chitosan can affect the activity normalized for lipase loading, and beads with desirable qualities can be produced.

Keywords
Chitosan; Lipase; Hydrogel beads; Candida rugosa; Hierarchical regression analysis
First Page Preview
Molecular weight and degree of deacetylation effects on lipase-loaded chitosan bead characteristics
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 17, September 2002, Pages 3637–3644
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering