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Cytotoxicity and MMP-9 activation induced in human mononuclear cells by UHMWPE oxidation

Paper ID Volume ID Publish Year Pages File Format Full-Text
13306 849 2002 6 PDF Available
Title
Cytotoxicity and MMP-9 activation induced in human mononuclear cells by UHMWPE oxidation
Abstract

Ultra high molecular weight polyethylene (UHMWPE) used in orthopedic prosthesis can be oxidized during sterilization processes such as γ-ray irradiation. Oxidation alters UHMWPE structure and mechanical properties and it has been suggested that this alteration is the first step in the loosening process. Direct effects of UHMWPE oxidation on the cellular and tissue response to the implant have not been previously investigated. We used heat-oxidized UHMWPE, whose oxidative state is comparable to the one induced in γ-ray irradiated polymer, in order to observe the peripheral blood mononuclear cells (PBMCs) behavior after 24 and 48 h exposure to the oxidized and non-oxidized polymers. Flow cytometric analysis showed a cytotoxic effect of oxidized UHMWPE after 48 h compared with the control samples. Moreover, gelatin zymography of PBMCs conditioned media showed a strong increase in MMP-9 (gelatinase B) release and activation in oxidized UHMWPE samples. This first evidence of a direct effect of the UHMWPE oxidative status on the cellular behavior suggests that oxidation alters not only the UHMWPE physical–mechanical properties, but it can also be responsible for altered tissue response.

Keywords
Oxidation; UHMWPE; PBMC; Cytotoxicity; MMP-9
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Cytotoxicity and MMP-9 activation induced in human mononuclear cells by UHMWPE oxidation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 17, September 2002, Pages 3645–3650
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us