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Enhanced fibrin remodeling in vitro with TGF-β1, insulin and plasmin for improved tissue-equivalents

Paper ID Volume ID Publish Year Pages File Format Full-Text
13313 849 2002 15 PDF Available
Title
Enhanced fibrin remodeling in vitro with TGF-β1, insulin and plasmin for improved tissue-equivalents
Abstract

The aim of this study was to better understand how to increase collagen content in and enhance mechanical properties of tissue-equivalents formed by entrapping tissue cells in fibrin gels, with the ultimate goal of developing mechanically robust artificial tissues. The two main areas of focus were cell culture medium composition and replacement frequency relative to a base case of incubating constructs in medium supplemented with just serum and replaced weekly. The optimal condition involved a combination of all three factors investigated—TGF-β1, insulin, plasmin—with medium replacement three times a week. Compared to a base case without these three factors, the combination case resulted in 20 times more collagen and a ten-fold increase in tensile strength. The high strain modulus and tensile strength were within an order of magnitude of cardiovascular tissues. This study indicates great potential for fibrin-based tissue-equivalents in soft connective tissue applications.

Keywords
Tissue engineering; Fibrin gel; Collagen synthesis; Fibroblast; TGF-beta; Insulin; Plasmin
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Enhanced fibrin remodeling in vitro with TGF-β1, insulin and plasmin for improved tissue-equivalents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 17, September 2002, Pages 3717–3731
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us