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The effect of the degree of chitosan deacetylation on the efficiency of gene transfection

Paper ID Volume ID Publish Year Pages File Format Full-Text
13328 850 2004 9 PDF Available
Title
The effect of the degree of chitosan deacetylation on the efficiency of gene transfection
Abstract

Chitosans with various degrees of deacetylation were synthesized by acetylation with acetic anhydride. These chitosans were evaluated for efficacy of nanoparticle formation, DNA binding efficiency, morphology, and in vitro and in vivo gene transfection efficiency. DNA binding efficacy was reduced as degree of deacetylation was decreased, therefore requiring an increased +/−ratio to effect complete DNA complexation. For chitosan with a molecular weight of 390 kDa, the +/−ratio to achieve complete DNA complexation for degrees of deacetylation of 90%, 70% and 62% was 3.3:1, 5.0:1, and 9.0:1, respectively. The size and morphology of these nanoparticle formulations were not significantly different. The decreased degree of deacetylation results in a decrease in overall luciferase expression levels in HEK293, HeLa, and SW756 cells due to particle destabilization in the presence of serum proteins. However, intramuscular luciferase expression levels increased with decreasing deacetylation over the time points tested. Degree of chitosan deacetylation is an important factor in chitosan–DNA nanoparticle formulation as it affects DNA binding, release and gene transfection efficiency in vitro and in vivo.

Keywords
Chitosan; DNA; Nanoparticles; Gene expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 22, October 2004, Pages 5293–5301
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us