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RGD-peptides for tissue engineering of articular cartilage

Paper ID Volume ID Publish Year Pages File Format Full-Text
13350 851 2002 9 PDF Available
Title
RGD-peptides for tissue engineering of articular cartilage
Abstract

One keypoint in the development of a biohybrid implant for articular cartilage defects is the specific binding of cartilage cells to a supporting structure. Mimicking the physiological adhesion process of chondrocytes to the extracellular matrix is expected to improve cell adhesion of in vitro cultured chondrocytes. Our approach involves coating of synthetic scaffolds with tailor-made, cyclic RGD-peptides, which bind to specific integrin receptors on the cell surface. In this study we investigated the expression pattern of integrins on the cell surface of chondrocytes and their capability to specifically bind to RGD-peptide coated materials in the course of monolayer cultivation. Human chondrocytes expressed integrins during a cultivation period of 20 weeks. Receptors proved to be functionally active as human and pig chondrocytes attached to RGD-coated surfaces. A competition assay with soluble RGD-peptide revealed binding specificity to the RGD-entity. Chondrocyte morphology changed with increasing amounts of cyclic RGD-peptides on the surface.

Keywords
Surface modification; RGD-peptide; FACS; Adhesion; Chondrocyte; Cartilage
First Page Preview
RGD-peptides for tissue engineering of articular cartilage
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 16, August 2002, Pages 3455–3463
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering