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Positively charged biomaterials exert antimicrobial effects on gram-negative bacilli in rats

Paper ID Volume ID Publish Year Pages File Format Full-Text
13364 852 2003 4 PDF Available
Title
Positively charged biomaterials exert antimicrobial effects on gram-negative bacilli in rats
Abstract

Biomaterial-centered infection is a much-dreaded complication associated with the use of biomedical implants. Although positively charged biomaterial surfaces stimulate bacterial adhesion, it has been suggested that surface growth of adhering Gram-negative bacilli is inhibited on positively charged surfaces. In the present paper, we determined the infection rate of differently charged poly(methacrylates) in rats. To this end, 2×106/cm2Escherichia coli O2K2 or 2×104/cm2Pseudomonas aeruginosa AK1 were seeded on glass discs coated with three differently charged poly(methacrylates) coatings in a parallel plate flow chamber. Three rats received six subcutaneous discs (two discs of each charge variant) seeded with E. coli, while three other rats received discs seeded with P. aeruginosa. The numbers of viable bacteria on the surfaces were determined 48 h after implantation. On 50% of all positively charged discs viable E. coli were absent, while the negatively charged discs were all colonized by E. coli. P. aeruginosa, however, were isolated from both positively and negatively charged discs. Probably, P. aeruginosa can circumvent the antimicrobial effect of the positive charge through the formation of extracellular polysacharides.

Keywords
Biomaterial-centered infection; E. coli; Pseudomonas aeruginosa; Rat model; Antimicrobial biomaterials
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Positively charged biomaterials exert antimicrobial effects on gram-negative bacilli in rats
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 16, July 2003, Pages 2707–2710
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us