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Investigation of recombinant human elastin polypeptides as non-thrombogenic coatings

Paper ID Volume ID Publish Year Pages File Format Full-Text
13380 853 2004 11 PDF Available
Title
Investigation of recombinant human elastin polypeptides as non-thrombogenic coatings
Abstract

We investigated the use of a recombinant human elastin polypeptide as a coating on synthetic materials with a view to determining if these polypeptides could improve the blood compatibility of cardiovascular devices such as vascular conduits and arterial/venous catheters. Platelet adhesion and activation were studied in vitro using three commercially available synthetic materials: polyethylene terephthalate (Mylar™), a poly(tetrafluoroethylene/ethylene) copolymer (Tefzel™) and a polycarbonate polyurethane (Corethane™). Coated with adsorbed polypeptide, all three synthetic materials demonstrated reduced platelet activation and adhesion in platelet rich plasma in vitro. Compared to non-coated controls, there was a significant decrease (p=0.05) in both platelet microparticle release and P-selectin expression for the polypeptide-coated surfaces. Scanning electron microscopy indicated fewer adhering platelets on coated surfaces compared to non-coated controls. In vivo, in a rabbit model, evaluations of polyurethane catheters coated with the polypeptide showed a marked increase in catheter patency and a significant decrease in fibrin accretion and embolism when compared to uncoated controls. This polypeptide shows a strong potential for use as a non-thrombogenic coating for small diameter vascular grafts. In addition, the results of this study indicate that the elastin polypeptide would be a valuable component of a tissue engineered vascular conduit.

Keywords
Elastin polypeptides; Non-thrombogenic coatings; Platelet activation; Platelets
First Page Preview
Investigation of recombinant human elastin polypeptides as non-thrombogenic coatings
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 19, August 2004, Pages 4543–4553
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering