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Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
13383 853 2004 8 PDF Available
Title
Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro
Abstract

Aim of this study was to investigate the effects of the resin monomer BisGMA on the glutathione concentration (monobromobimane assay) and apoptosis (Annexin V/PI-assay) of cultured primary human gingival fibroblasts. Cells were treated for up to 24 h with 0.001–0.25 mm BisGMA to determine growth curves using the DNA stain H33342. Subsequent Annexin V/PI-assays revealed that fibroblasts exposed to concentrations of 0.005–0.01 mm (non-cytotoxic) and 0.05 mm (ED10-concentration) showed no increase of the share of apoptotic cells compared to non-treated controls (5–8%), while 0.1 mm BisGMA (∼ ED50-concentration) caused a significant increase of the percentage of apoptotic cells (50%). Simultaneously to the induction of apoptosis, 0.1 and 0.25 mm of BisGMA caused a significant depletion of the intracellular GSH content after 18 h of incubation. Our results indicate that BisGMA at concentrations >0.1 mm causes an extreme depletion of the intracellular GSH pool as well as apoptosis.

Keywords
Gingival fibroblasts; BisGMA; Apoptosis; Glutathione
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Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 19, August 2004, Pages 4573–4580
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us