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A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4), on titanium alloy

Paper ID Volume ID Publish Year Pages File Format Full-Text
13434 855 2002 9 PDF Available
Title
A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4), on titanium alloy
Abstract

Immobilization of biomolecules on surfaces enables both localization and retention of molecules at the cell–biomaterial interface. Since metallic biomaterials used for orthopedic and dental implants possess a paucity of reactive functional groups, biomolecular modification of these materials is challenging. In the present work, we investigated the use of a plasma surface modification strategy to enable immobilization of bioactive molecules on a “bioinert” metal. Conditions during plasma polymerization of allyl amine on Ti–6Al–4V were varied to yield 5 (“low”)- and 12 (“high”)-NH2/nm2. One- and two-step carbodiimide schemes were used to immobilize lysozyme, a model biomolecule, and bone morphogenetic protein-4 (BMP-4) on the aminated surfaces. Both schemes could be varied to control the amount of protein bound, but the one-step method destroyed the activity of immobilized lysozyme because of crosslinking. BMP-4 was then immobilized using the two-step scheme. Although BMP bound to both low- and high-NH2 surfaces was initially able to induce alkaline phosphatase activity in pluripotent C3H10T1/2 cells, only high amino group surfaces were effective following removal of weakly bound protein by incubation in cell culture medium.

Keywords
Protein immobilization; Plasma polymerization; Carbodiimide; BMP; Biomolecular modification
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A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4), on titanium alloy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 9, May 2002, Pages 2079–2087
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us