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Smooth muscle-like tissues engineered with bone marrow stromal cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
13465 858 2004 8 PDF Available
Title
Smooth muscle-like tissues engineered with bone marrow stromal cells
Abstract

Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM α-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell–scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM α-actin and SM myosin heavy chain. Masson’s trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM.

Keywords
Bone marrow stromal cells; Smooth muscle; Tissue engineering; Biodegradable polymer scaffold
First Page Preview
Smooth muscle-like tissues engineered with bone marrow stromal cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 15, July 2004, Pages 2979–2986
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering