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PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment

Paper ID Volume ID Publish Year Pages File Format Full-Text
13469 858 2004 9 PDF Available
Title
PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment
Abstract

The aim of this study was to examine the effect of increased content of 45S5 Bioglass® (0–40 wt%) in poly(dl-lactic acid) (PDLLA) porous foams on the behaviour of MG-63 (human osteosarcoma cell line) and A549 cells (human lung carcinoma cell line). The ability of these cell lines to grow on bioactive composites was quantitatively investigated in order to assess the potentiality for their use in hard and soft-tissue engineering. Two hours after cell seeding, an increase of cell adhesion according to the increased content of Bioglass® present in the foams for both cell types was observed. Cell proliferation studies performed over a period of 4 weeks showed a better aptitude of the A549 cells to proliferate on PDLLA foams containing 5 wt% Bioglass® when compared to the proliferation on foams with 40 wt% Bioglass®. A lower proliferation rate was obtained for cells on pure PDLLA. Scanning electron microscopy analysis showed for both cell types the presence of cells inside the porous structure of the foams. These results confirmed the biocompatibility of PDLLA/Bioglass® composite foams and the positive effect of Bioglass® on MG-63 cell behaviour and also showed for the first time the possibility for human lung epithelial type II cells to adhere and proliferate on these porous scaffolds. In addition, we describe a positive effect of 45S5 Bioglass® on A549 cell behaviour in a dose-dependent manner, indicating the potential of using PDLLA/Bioglass® composites with an optimal concentration of 45S5 Bioglass® not only in bone tissue engineering but also in lung tissue engineering.

Keywords
Bone tissue engineering; Lung; Epithelial cells; Polylactic acid; Bioactive glasses
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PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 15, July 2004, Pages 3013–3021
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us