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Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
13480 858 2004 8 PDF Available
Title
Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan ☆
Abstract

Schizophyllan is a β-(1→3)-d-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis–Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides.

Keywords
DNA; Enzyme; Hydrolysis; Polysaccharide
First Page Preview
Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan ☆
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 15, July 2004, Pages 3109–3116
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering