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Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
13481 858 2004 7 PDF Available
Title
Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis ☆
Abstract

Schizophyllan is a β-(1→3)-d-glucan and can form a novel complex with some single-chains of DNAs. As the preceding paper revealed, the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide). This paper examined possibility to apply this complex to an antisense DNA carrier, using an in vitro (cell-free) transcription/translation assay. In this assay, we used a plasmid DNA coding a green fluorescence protein (GFP) and an antisense DNA designed to hybridize the ribosome-binding site in the GFP-coded mRNA. When the antisense DNA was administered as the complex, a lower GFP expression efficiency (or higher antisense effect) is observed over naked DNA. This is because the antisense DNA in the complex is protected from the attack of deoxyribonuclease. When exonuclease I, which specifically hydrolyzes single DNA chains, was present in the GEP assay system, the antisense effect was not changed for the complex while being weakened in the naked antisense DNA system. These results imply that the exonuclease I cannot hydrolyze the antisense DNA in the complex, while it can hydrolyze naked DNA to reduce its antisense effect.

Keywords
Antisense; DNA; Gene expression; Polysaccharide; Complexation
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Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 15, July 2004, Pages 3117–3123
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us