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Pegylated recombinant human epidermal growth factor (rhEGF) for sustained release from biodegradable PLGA microspheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
13500 861 2002 7 PDF Available
Title
Pegylated recombinant human epidermal growth factor (rhEGF) for sustained release from biodegradable PLGA microspheres
Abstract

Recombinant human epidermal growth factor (rhEGF) was conjugated with polyethylene glycol (PEG) to improve its physical stability during microencapsulation in biodegradable poly(lactic-co-glycolic acid) microspheres. rhEGF was conjugated with N-hydroxysuccimide (NHS)-derivatized methoxy-PEG (mPEG) of MW 2000 and 5000 under various reaction conditions to optimize the extent of pegylation. Pegylated rhEGF showed much enhanced physical stability against homogenization. Pegylated rhEGF was encapsulated in PLGA microspheres by a double emulsion solvent evaporation method to achieve a sustained release. Pegylated rhEGF exhibited a tri-phasic release profile with a reduced initial burst, compared with unpegylated rhEGF. This study demonstrated that protein pegylation enhanced physical stability of protein and could be a good approach to achieve a sustained protein release profile from biodegradable microspheres.

Keywords
Recombinant human epidermal growth factor (rhEGF); Pegylation; PLGA; Microspheres; Stability
First Page Preview
Pegylated recombinant human epidermal growth factor (rhEGF) for sustained release from biodegradable PLGA microspheres
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 11, June 2002, Pages 2311–2317
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering