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The stiffness of bone marrow cell–knit composites is increased during mechanical load

Paper ID Volume ID Publish Year Pages File Format Full-Text
13539 876 2001 10 PDF Available
Title
The stiffness of bone marrow cell–knit composites is increased during mechanical load
Abstract

A novel device for mechanical stimulation of primary adult rat bone marrow cells cultured on three-dimensional knitted textiles has been prototyped. A method has been developed ensuring a well-defined, high-density, and reproducible cell seeding on the knitted fabric. After culturing for 18–52 days the cell–knit composites were subjected to uniaxial 2% stretching and relaxation. The frequency was altered between 0.1 Hz (196 min, loading phase) and 0.01 Hz (360 min, resting phase). Identically treated knits without cells exhibited a slight stiffness reduction, whereas the stiffness of knits with cells increased from cycle to cycle. The stiffness increase was found to depend on the duration of the culture period before mechanical loading. Our data suggest that the extracellular matrix deposited by the cells on the knit and intact microtubuli of living cells cause the observed stiffness increase. In comparison to the unstrained static cell–knit composites cell proliferation and bone cell differentiation were reduced by the mechanical load.

Keywords
ALP: Alkaline phosphatase; RBMC: Rat bone marrow cells; PBS: Phosphate buffered saline; TRAP: Tartrate-resistant acid phosphataseMechanical load; Culture; Bone marrow; knit; Stiffness; Strain
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The stiffness of bone marrow cell–knit composites is increased during mechanical load
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 23, December 2001, Pages 3169–3178
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us