fulltext.study @t Gmail

Insulinotropic activity of sulfonylurea/pullulan conjugate in rat islet microcapsule

Paper ID Volume ID Publish Year Pages File Format Full-Text
13556 880 2003 9 PDF Available
Title
Insulinotropic activity of sulfonylurea/pullulan conjugate in rat islet microcapsule
Abstract

The in vitro long-term effect of a water-soluble sulfonylurea/pullulan conjugate (SUP) on insulinotropic activity and cell viability was investigated using rat pancreatic islets co-entrapped with SUP in conventional alginate-poly(l-lysine) microcapsules. The conjugate was synthesized by coupling a carboxylated glibenclamide derivative to a polysaccharide, pullulan (MW=200,000). In vitro static experiment showed that sulfonylurea concentration in SUP over 50 μm was required to stimulate the rat islets. In a dynamic insulin secretion test, the microcapsules of islets with SUP regained the insulin secretion pattern comparable to that of free islets, while those without SUP showed impaired insulin secretion. The long-term (1 month) culture experiment demonstrated that the microcapsules of islets with SUP, with well-preserved morphology, presented higher insulin secretion level and better ability in responding to glucose changes than those without SUP.

Keywords
Biohybrid artificial pancreas; Sulfonylurea; Polymeric insulinotropic agent; Islet; Insulin; Microcapsules
First Page Preview
Insulinotropic activity of sulfonylurea/pullulan conjugate in rat islet microcapsule
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 26, November 2003, Pages 4843–4851
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us