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Gelatin–chondroitin–hyaluronan tri-copolymer scaffold for cartilage tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
13557 880 2003 6 PDF Available
Title
Gelatin–chondroitin–hyaluronan tri-copolymer scaffold for cartilage tissue engineering
Abstract

The mechanism by which the cell synthesizes and secretes extracellular matrix (ECM) and is, in turn, regulated by the ECM is termed dynamic reciprocity. The aim of the present work was to produce a gelatin/chondoitin-6-sulfate/hyaluronan tri-copolymer to mimic natural cartilage matrix for use as a scaffold for cartilage tissue engineering. The scaffold produced had a uniform pore size of about 180 μm and adequate porosity of 75%. Porcine chondrocytes were seeded onto the tri-copolymer scaffold and cultured in Petri dishes or spinner flasks for 2, 3, 4, or 5 weeks. Chondrocytes were uniformly distributed in the scaffold in the spinner flask cultures, but less so in the Petri dish cultures. Secretion of ECM was found under histology examination. In spinner flask cultures, chondrocytes retained their phenotype for at least 5 weeks, as shown immunohistochemically, and synthesized type II collagen. These results show that gelatin/chondroitin sulfate/hyaluronan tri-copolymer has potential for use as a cartilage tissue engineering scaffold.

Keywords
Gelatin; Chondroitin sulfate; Hyaluronan; Cartilage; Tissue engineering
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Gelatin–chondroitin–hyaluronan tri-copolymer scaffold for cartilage tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 26, November 2003, Pages 4853–4858
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us