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Study of a (trimethylenecarbonate-co-ε-caprolactone) polymer—Part 2: in vitro cytocompatibility analysis and in vivo ED1 cell response of a new nerve guide

Paper ID Volume ID Publish Year Pages File Format Full-Text
13563 881 2001 8 PDF Available
Title
Study of a (trimethylenecarbonate-co-ε-caprolactone) polymer—Part 2: in vitro cytocompatibility analysis and in vivo ED1 cell response of a new nerve guide
Abstract

Future surgical strategies to restore neurological function in peripheral nerve loss may involve replacement of nerve tissue with cultured Schwann cells using biodegradable guiding implants. Random copolymers of trimethylene carbonate and ε caprolactone (P(εCL-TMC), 50 : 50) have been synthesized by ring opening polymerization using rare earth alkoxides as initiator. Their potential use as nerve guide repairs has been assessed through indirect and direct in vitro biocompatibility tests and in vivo soft tissue response to ED1 subclass macrophages. In vitro, we exposed monolayers of human skin fibroblasts and an established continuous cell line (Hela) to liquid extracts (either pure or diluted in the culture medium) of εCL-TMC copolymer including positive (phenol) and negative controls. Then, colorimetric assays (Neutral red and MTT) were performed. The extracts of εCL-TMC induced no significant cytotoxic effect. We also exposed in vitro Schwann cells to pieces of P(εCL-TMC) and P(LA-GA) copolymers. We evaluated cell attachment at 1 and 3 h by measuring the activity of the lysosomal enzyme (N-acetyl-β-hexosaminidase) and cell proliferation at 1, 3, 6 and 9 days by measuring the cell metabolic activity (MTT assay). Values for attachment slightly decreased between 1 and 3 h but were significantly higher than on agars (negative control). Cells plated on εCL-TMC showed a rate of proliferation comparable with that of normalized controls and higher than on PGA-PLA at day 9. Finally, we evaluated in vivo the soft tissue response after implantation of cylindrical tubes of P(εCL-TMC) and P(LA-GA) copolymers with an immunohistochemistry staining procedure for the newly recruited ED1 macrophages. An image analysis system automatically measured the optical density of labelled positive ED1 cells at 9, 21 and 60 days after implantation. εCL-TMC copolymer showed a mild soft tissue reaction with no adverse chronic inflammatory reaction. These data allowed us to consider this conduit as a potential effective substitute in nerve repair.

Keywords
ε-Caprolactone; Trimethylene carbonate; Nerve guide; Biocompatibility evaluation
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Study of a (trimethylenecarbonate-co-ε-caprolactone) polymer—Part 2: in vitro cytocompatibility analysis and in vivo ED1 cell response of a new nerve guide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 22, 15 November 2001, Pages 2951–2958
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us